"Determination of polyphenolic compounds accumulated in the leaves and tubers of Crocus sativus L. and determination of antiradical activity"

B. Marciuškaitė master’s thesis, title "Determination of polyphenolic compounds accumulated in the leaves and tubers of Crocus sativus L. and determination of antiradical activity". Science manager doc. K. Vitkevičius; Department of Analytical and Toxicological Chemistry at the Faculty of Pharmacy, Lithuanian University of Health Sciences. Kaunas, 2020. Key words: Crocus sativus L., phenolic acids, flavonoids, corms, leaves, antiradical activity. The aim of the research: to identify and compare the flavonoids and phenolic compounds accumulated in the leaves and corms of Crocus sativus L. and to evaluate their antiradical activity. The tasks of the research: to investigate the diversification of flavonoids and phenolic compounds content and antiradical activity in leaf and corm samples of Crocus sativus L. The object of the research: Crocus sativus L. leaves and corms from Ukraine, Kharkiv, Karazina; Ukraine, Nikolaev; Ukraine, Kherson Oblast, Liubimovka. The methods of the research: total flavonoids and phenolic compounds were determined spectrophotometrically. Antiradical activity was determined by ABTS radical-cation binding method, spectrophotometrically. The results of the research: the total amount of flavonoids in the samples of leaves and corms of Crocus sativus L. from different regions of Ukraine varied from 2,96 ± 0,21 µmol/g to 32,9 ± 1,97 µmol/g (p<0,05), the total amount of phenolic compounds Crocus sativus L. in leaf and corm samples from different regions of Ukraine varied from 3,34 ± 0,07 µmol/g to 19,08 ± 1,53 µmol/g (p<0,05). The antiradical activity values of leaves and corms extracts of Crocus sativus L. ranged from 80,5 ± 3,22 μmol/g to 1362,58 ± 68,13 μmol/g (p<0,05). The conclusion of the research: optimal extraction conditions for the determination of phenolic compounds and flavonoids: solvent – 70 % ethanol, extraction time – 20 min. The highest amounts of flavonoids and antiradical activity were found in the samples of Crocus sativus L. leaves. There was a correlation between the amount of flavonoids and phenolic compounds and antiradical activity (p